Ōtani Yos-tsugu (大谷 吉継, 1558 or 1565 – October 21, 1600) was a Japanese samurai of the Sengoku period through the Azuchi-Momoyama Period. He was also known by his court title Junior Assistant Minister of Justice or Gyōbu-shōyū (刑部少輔). He was born in 1558 to a father who was said to be a retainer of either Ōtomo Sōrin or Rokkaku Yoshikata. He became one of Toyotomi Hideyoshi's followers. He participated in the Toyotomi's Odawara campaign and Korean campaign.
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