1. Wario Wario, Waluigi Wario, Pauline, Wendy O. Koopa, Lou Albano, Bird and Kamek were in this film instead of Spike, Iggy, Daniella Verducci, Lena, Big Bertha, Anthony Scapelli and Sergeant Simon.
2. Toad is short wearing a Mushroom hat.
3. King Koopa is a fire-breathing Koopa that based on the Video Game character instead of a human.
4. They sucked in the Warp Pipe to Dinohatten instead of through the wall.
5. They used their catchphrase like "It's-a-me, Mario!", "Mamma Mia!", "Here we go!", "Waaaaahhhh!", "Mario time!", "Okey dokey!", "Wahoo!", and "Let's-a go!"
