After a few years since the tragic death of Gwen Stacy, Peter Parker (Tobey Maguire) tries to balance his life as Spider-Man and his family responsibilities with Mary Jane (Kirsten Dunst), whom he has always loved and who are now married. The couple has a daughter, Mayday Parker, who grows up amid the complexities of her father's life as a superhero.
Meanwhile, Elizabeth Toomes (Angelina Jolie), recently released from prison, devises a cunning plan to form a Sinister Six, made up of villains captured by Spider-Man, such as Mystery, Rhino and Scorpion and some new members such as the dangerous Cletus Kasady and Max Dillon. To manipulate Roderick Kingsley into joining the team, Elizabeth reveals Spider-Man's true identity, revealing that he is her own friend Peter, further intensifying the conflict between the two.
The movie explores the complex dynamics between Peter and the villains he has faced over the years, now united against him and the confrontation with Roderick now as Hobgoblin, who continued with Oscorp's enhancement serum project believing it could work. As Mayday grows and becomes increasingly involved in her father's life as a hero, Peter faces moral dilemmas, shocking revelations and emotional challenges.
